Contact the University of Washington Clinical Virology Lab (206 685-8037) for ordering instructions. CSF antibody testing will be performed ONLY when paired with a serum sample. For accurate seroconversion determination, the acute and convalescent samples should be drawn at least 12 -16 weeks apart.įor CSF, see HSV SemiQnt Rapid PCR, Swab/CSF (Herpes Simplex Virus by PCR). Committee of the Southern Illinois University School of Medicine. The detection of HSV1 and HSV2 IgG class antibodies by Western blot in serum or CSF.
#UNIVERSITY OF WASHINGTON HSV WESTERN BLOT CODE#
It has not been cleared or approved by the US Food and Drug Administration.įor Paired samples, see Herpes Simplex Seroconversion Panel by Western Blot (Paired) for seroconversion on paired serum samples. Western blot analyses indicated the live HSV-2 0NLS vaccine elicited an IgG antibody. HSV Western Blot Lab Code HSWB Epic Ordering Herpes Type 1 and 2 Serology Description. This test was developed and its performance characteristics determined by the University of Washington Department of Laboratory Medicine and Pathology. If you are from a clinical facility outside the UW Medicine system and have specific questions about laboratory specimens call Reference Laboratory Services at: Monday Friday 7:00 AM 11:00 PM, Saturday 8:00 AM 3:30 PM (PST) Tel: (206) 520-4600 or 1 (800) 713-5198. HSV2 - Positive, Negative or Indeterminate Special requests for testing should be noted on the requisition.HSV1 - Positive, Negative or Indeterminate.The pattern of staining on the two blots (HSV-1 and HSV-2) is dictated by the number and identity of the HSV proteins to which the patient's immune system has antibody. Women with and without known HIV infection who delivered at the University of Washington Medical Center between 19891996 had HSV serologies done as part of. Antibodies, which bind to the viral proteins, are detected by an enzyme-mediated color change. with western blot (WB) as the final arbiter of HSV serostatus. The other expenses you will incur to get this test is a fee for having your blood drawn and whatever shipping costs to UW from your location. 1Department of Laboratory Medicine, University of Washington and 2Childrens Hospital and. I will charge your credit card the appropriate amount at the end of our discussion. The strips of paper or "blots" containing separated fixed proteins from either HSV-1 or HSV-2 are incubated with the patient's serum. The cost of obtaining the western blot from me is 325. HSV1 and HSV2 proteins from detergent lysates ("Bernstein's lysate") of HSV infected cells are separated by electrophoresis and transferred to nitrocellulose paper.
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